The turnover number for band 3‐mediated sulfate transport in phosphatidylcholine bilayers

FEBS Letters

Lindenthal, Sabine¹  Grieshaber, Gabriele¹  Haase, Winfried¹  Schubert, Dieter¹  Scheuring, Uwe¹ 

[1] Max-Planck-Institut für Biophysik, Frankfurt am Main, FRG

关键词: Band 3 protein;    Sulfate transport;    Reconstitution;    Turnover number;    (Erythrocyte membrane);    PC;    phosphatidylcholine;    H2DIDS;    4;    4′-diisothiocyanostilbene-2;    2′-disulfonate;   

DOI  :  10.1016/0014-5793(88)81407-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The anion transport system of the human erythrocyte membrane was reconstituted in unilamellar phosphatidylcholine vesicles, and a vesicle subpopulation of a narrow size distribution was isolated from the sample by gel filtration. In this subpopulation, the turnover number of the transport protein (the band 3 protein) for sulfate transport was determined. It was found that, in the reconstituted system, the protein transports sulfate 5–10-times faster than in the human erythrocyte membrane.

【 授权许可】

Unknown