Preformed PAF‐acether and lyso PAF‐acether are bound to blood lipoproteins

FEBS Letters

Bidault, Jocelyne¹  Benveniste, Jacques¹  Korth, Ruth¹  Nunez, Daniele¹  Fruchart, Jean-Charles²  Duriez, Patrick² 

[1] INSERM U 200, Université Paris-Sud, 32 rue des Carnets, 92140 Clamart, France;SERLIA, Institut Pasteur, 1 rue Calmette, 59000 Lille, France

关键词: PAF-acether;    Lyso PAF-acether;    Lipoprotein;    ADP;    adenosine diphosphate;    HDL;    high density lipoprotein;    HPLC;    high-pressure liquid chromatography;    LDL;    low density lipoprotein;    Ls;    lipoproteins;    PC;    phosphatidylcholine;    rt;    retention time;    VLDL;    very low density lipoprotein;   

DOI  :  10.1016/0014-5793(88)81456-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PAF-acether (PAF) is a newly formed mediator not normally present in circulating blood. A compound exhibiting all of its biological characteristics but coeluting with phosphatidylcholine (PC) in high-pressure liquid chromatography (HPLC) was unveiled (‘peak X’) in normal human plasma. A second HPLC run of peak X HPLC fractions revealed the presence of PAF itself with concomitant disappearance of peak X. Beside PAF, immunoreactive apolipoproteins A-I and E were found in peak X. Also lipoproteins (Ls) purified using either ultracentrifugation or immunoaffinity chromatography yielded peak X and, in a second HPLC run, authentic PAF. L-free plasma was devoid of peak X. Finally, after preincubation with plasma, labeled PAF was found associated with Ls. Thus in human blood preformed PAF is bound in high amounts to Ls, a result of interest given the role of Ls and platelets in vascular diseases and the present knowledge on PAF biosynthesis.

【 授权许可】

Unknown