Sequences both 5′ and 3′ to the transcription initiation site contribute to the ability of a mouse H‐2 gene to respond to type I interferon

FEBS Letters

Pannuti, Antonio¹  Lania, Luigi¹  Mantia, Girolama La¹  Pascucci, Anna¹ 

[1] Dipartimento di Genetica, Biologia Generale e Molecolare, University of Naples, Via Mezzocannone 8, 80134 Naples, Italy

关键词: Interferon;    H-2 gene transcription;    Gene regulation;   

DOI  :  10.1016/0014-5793(88)81443-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate the cis-acting DNA elements that are involved in the regulation of class I major histocompatibility complex genes by interferon, several promoter fragments of the H-2K^k gene were linked to the reporter chloramphenicol acetyl transferase (CAT) gene, and the CAT expression was analyzed in stable transfected cell lines. The functional activities of progressive deletions of the 5′-flanking region of the H-2K^k gene linked to the CAT gene have allowed us to define a discrete cis-acting DNA region necessary for interferon-mediated stimulation. Moreover, the H-2K^k gene transcribed by the nonregulated SV40 early promoter was also found to be under interferon regulation. Thus interferon enhancement of the H-2K^k gene expression appears to be mediated by two cis-acting elements, one located in the 5′-flanking region and the other by sequences downstream from the transcription initiation site.

【 授权许可】

Unknown