Low temperature spectrophotometry on the photoreaction cycle of sensory rhodopsin

FEBS Letters

Ariki, Masahiro¹  Yoshizawa, Tôru¹  Shichida, Yoshinori¹ 

[1]Department of Biophysics, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606, Japan

关键词: Sensory rhodopsin;    Photointermediate;    Photoreaction;    Potential barrier;    Activation energy;    Low-temperature spectrophotometry;    (Halobacterium halobium);    Mops;    3-(N-morpholino)propanesulfonic acid;   

DOI  :  10.1016/0014-5793(87)81169-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The photoreaction of sensory rhodopsin (sR) was studied by using low temperature spectrophotometry. Upon irradiation of sR₅₉₀ at temperatures ranging from −30 to −60°C, sR₃₇₃, a photointermediate was produced without any preceding photoproducts. The formation of sR₃₇₃ was temperature-dependent, and an Arrhenius plot gave an activation energy of 15.7 kcal/mol, suggesting that there might be a potential barrier in the excited state of sR₅₉₀.

【 授权许可】

Unknown