Reconstitution of isolated Rieske Fe‐S protein into a Rieske‐depleted cytochrome b 6‐ƒ complex

FEBS Letters

Adam, Zach¹  Malkin, Richard¹ 

[1] Division of Molecular Plant Biology, University of California, Berkeley, CA 94720, USA

关键词: Cytochrome b 6-ƒ;    Rieske Fe-S protein;    Reconstitution;    BCA;    bicinchoninic acid;    CMC;    critical micellar concentration;    Mes;    2-(N-morpholino)ethane-sulfonic acid;   

DOI  :  10.1016/0014-5793(87)81132-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Rieske Fe-S protein can be isolated from the cytochrome b ₆-ƒ complex by means of chromatography on a hydroxyapatite column in the presence of detergent. Depletion of the cytochrome complex from the Rieske protein results in the loss of oxidoreductase activity, as well as the ability to reduce cytochrome b ₆. The Rieske Fe-S protein can be reconstituted into the Rieske-depleted complex by removal of the Triton X-100 molecules associated with the protein fractions, and their substitution by lipids. Upon reconstitution the complex is reactivated, and the role of the Rieske Fe-S protein in the reduction of both plastocyanin and cytochrome b ₆ can be demonstrated.

【 授权许可】

Unknown