Kinetics of pump currents generated by the Na+,K+‐ATPase

FEBS Letters

Grell, E.¹  Bamberg, E.¹  Fendler, K.¹ 

[1]Max-Planck-Institut für Biophysik, Heinrich-Hoffmann-Str. 7, D-6000 Frankfurt am Main, FRG

关键词: Na+;    K+-ATPase;    Black lipid membrane;    Transient kinetics;    Electrogenic property;    Pump current;   

DOI  :  10.1016/0014-5793(87)80427-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Purified Na⁺,K⁺-ATPase from pig kidney was attached to black lipid membranes. Pump currents of the enzyme could be measured with a time resolution of approx. 1 ms by releasing ATP from caged ATP with a UV laser flash. Analysis of the transient currents shows that a slow non-electrogenic step is followed by an electrogenic transition with a rate constant of 100 s⁻¹ (22°C). The exponential components found in the transient currents are compared to transitions in the Albers-Post scheme.

【 授权许可】

Unknown