Labeling of human erythrocyte membrane proteins by photoactivatable radioiodinated phosphatidylcholine and phosphatidylserine A search for the aminophospholipid translocase

FEBS Letters

Hervé, Paulette¹  Fellmann, Pierre¹  Zachowski, Alain¹  Devaux, Philippe F.¹ 

[1] Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, F-75005 Paris, France

关键词: Phospholipid flip-flop;    Nitrene;    Erythrocyte;    Spin-label;    ESR;    BSA;    bovine serum albumin;    ESR;    electron spin resonance;    PS;    phosphatidylserine;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(87)80311-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have synthesized radioiodinated photoactivatable phosphatidylcholine (¹²⁵-N₃-PC) and phosphatidylserine (¹²⁵I-N₃-PS). After incubation with red blood cells in the dark, the labeled PC could be extracted but not the corresponding PS molecule, indicating that the latter was transported by the aminophospholipid translocase, but not the former. When irradiated immediately after incorporation, N₃-PS, but not N₃-PC, partially blocked subsequent translocation of spin-labeled aminophospholipids. Analysis of probe distribution by SDSpolyacrylamide gel electrophoresis revealed that ¹²⁵I-N₃-PS labeled seven membrane bound components with molecular masses between 140 and 27 kDa: one (or several) of these components should correspond to the aminophospholipid translocase.

【 授权许可】

Unknown