Vertical fluctuations of phospholipid acyl chains in bilayers

FEBS Letters

Ghiggino, Kenneth P.¹  Sawyer, William H.¹  Wardlaw, John R.¹ 

[1] Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, Australia

关键词: Bilayer dynamics;    Fluorescence quenching;    Anthroyloxy fatty acid;   

DOI  :  10.1016/0014-5793(87)80502-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possibility of vertical displacement of acyl chains in lipid bilayers has been examined by quenching the fluorescence of 2-(9-anthroyloxy)palmitic acid with 5- and 16-doxylstearates in dipalmitoylphosphatidyl-choline unilamellar vesicles. Measurement of lifetime and steady-state quenching showed that the dynamic component of quenching was independent of the transverse position of the quencher indicating that a quencher at the 16-position could interact with a fluorophore at the 2-position with high frequency. The differences in steady-state quenching could be accounted for by the differences in the static component of quenching. The results provide further evidence for rapid vertical displacements of acyl chains in phospholipid bilayers.

【 授权许可】

Unknown