FEBS Letters | |
Transfer of functional opiate receptors from membranes to recipient cells by polyethylene glycol‐induced fusion | |
Klee, A.1  Tocqué, B.3  Pfeiffer, A.2  | |
[1] Laboratory of Molecular Biology, National Institute of Mental Health, 9000 Rockville Pike, Building 36, Room 1B-08, Bethesda, MD 20205 USA;Medizinische Klinik II, Ludwig-Maximilians-Universität, Klinikum Gross Hadern, Marchioninistr. 15, D-8000 München 70, FRG;>Rhône-Poulenc Santé, Centre de Recherches de Vitry, 13, quai Jules Guesde, 94403 Vitry-sur-Seine, France | |
关键词: Opiate receptor; Reconstitution; Membrane fusion; Adenylate cyclase; NEM; N-ethylmaleimide; PEG; polyethylene glycol; DALAMID; (D-Ala2)enkephalin(5-L-methioninamide); Mops; 4-morpholinepropanesulfonic acid; | |
DOI : 10.1016/0014-5793(87)80395-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
Opiate receptor-mediated inhibition of adenylate cyclase activity was elicited in membranes of C6BUI glioma cells and S49 cyc− lymphoma cells after fusion with opiate receptor-containing membranes derived from NG108-15 neuroblastoma × glioma hybrid cells. The fusion was induced by polyethylene glycol using procedures developed by Orly and Schramm [(1976) Proc. Natl. Acad. Sci. USA 73, 4410–4414]. Prior to fusion, the adenylate cyclase activity of the donor, NG108-15 cell membrane, was inactivated by N-ethylmaleimide treatment. Prostaglandin E1 receptors and the stimulatory GTP-binding protein Ns were transferred to the recipient cells along with opiate receptors. Thus, inhibitory receptors can be tranferred to foreign adenylate cyclase systems just as stimulatory receptors had earlier been found to do. Furthermore, opiate receptors have been shown to function in non-neuronal cells.
【 授权许可】
Unknown
【 预 览 】
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