Characterization of the free radical in a plant ribonucleotide reductase

FEBS Letters

Harder, Jens¹  Follmann, Hartmut¹ 

[1] Fachbereich Chemie, Arbeitsgruppe Biochemie der Philipps-Universität, Hans-Meerwein-Strasse, D-3550 Marburg, FRG

关键词: Ribonucleotide reductase;    EPR;    Hydroxyurea;    (Green alga);   

DOI  :  10.1016/0014-5793(87)80214-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ribonucleotide reductase of the green alga, Scenedesmus obliquus, contains an organic free radical in its catalytic subunit U2 which is quenched by hydroxyurea and regenerated upon aerobic incubation in the presence of Fe²⁺. The low-temperature EPR spectrum exhibits a doublet centered at g = 2.0046. This EPR signal is indistinguishable from that of the tyrosine radical in mouse cell ribonucleotide reductase, indicating that the eukaryotes possess one common enzyme apparatus for deoxyribonucleotide biosynthesis.

【 授权许可】

Unknown