ATP synthesis by the (Na+ + K+)‐ATPase in the absence of an ionic gradient Effects of organic solvent

FEBS Letters

de Moraes, Vera Lucia Goncalves¹  de Meis, Leopoldo¹ 

[1] Instituto de Ciencias Biomedicas, Departamento de Bioquimica, Universidade Federal do Rio de Janeiro, Ilha do Fundao, 21910 Rio de Janeiro, Brazil

关键词: ATP synthesis;    (Na+ + K+)-ATPase;    Organic solvent effect;   

DOI  :  10.1016/0014-5793(87)80212-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 200-fold decrease in the P_i concentration required for half-maximal phosphorylation of the (Na⁺ + K⁺)-ATPase is observed when 40% (v/v) dimethyl sulfoxide is added to the assay medium. The phosphoenzyme formed in the presence of dimethyl sufoxide is able to transfer its phosphate to ADP to form ATP when 400 mM NaCl is added to the medium. The synthesis of ATP depends on the concentrations of organic solvent and of Na⁺, and is inhibited by ouabain.

【 授权许可】

Unknown