Mitochondrial aldehyde dehydrogenase Homology of putative targeting sequence to that of carbamyl phosphate synthetase I revealed by correlation of cDNA and protein data

FEBS Letters

Höög, Jan-Olov²  Hempel, John¹  Jörnvall, Hans² 

[1] Department of Biochemistry, University of Pittsburgh, Pittsburgh, PA 15261, USA;Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden

关键词: Aldehyde dehydrogenase;    Carbamyl phosphate synthetase;    cDNA;    Frameshift;    Leader sequence;    Mitochondrial targeting;   

DOI  :  10.1016/0014-5793(87)80198-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Comparison of existing protein and cDNA data for human liver mitochondrial aldehyde dehydrogenase reveals deviations in two segments. They are shown to correspond exactly to localized frameshifts in the cDNA data, which are likely to have three reading errors. After correction of the cDNA frameshifts, a deduced amino acid sequence corresponds exactly to the data established at the protein level. In addition, extension of the shifted frame into the cDNA corresponding to the mitochondrial leader sequence allows reinterpretation of that sequence. The new leader sequence is consistent with characteristics of such segments of other mitochondrial protein pro-forms. Furthermore, the sequence displays a homology, when centered around the cleavage site, with the leader sequence of rat liver carbamyl phosphate synthetase I, suggesting a novel similarity between mitochondrial targeting sequences of two different enzymes.

【 授权许可】

Unknown