Developmental changes in the lateral diffusion of Leydig cell membranes measured by the FRAP method

FEBS Letters

Garland, P.B.¹  Birmingham, J.¹  Foley, M.¹  Zlatanov, I.V.² 

[1] Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland U.K.;Bulgarian Academy of Sciences, Central Laboratory of Biophysics, 1113 Sofia, Bulgaria

关键词: FRAP;    Fluorescent probe;    Lateral diffusion;    Age dependence;    (L-cell);   

DOI  :  10.1016/0014-5793(87)80189-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility of membrane lipids and proteins were discovered.

【 授权许可】

Unknown