Adenylate cyclase stimulating agents and mitogens raise fructose 2,6‐bisphosphate levels in human fibroblasts Evidence for a dual control of the metabolite

FEBS Letters

Farnararo, Marta¹  Bruni, Paola¹  Vasta, Valeria¹ 

[1] Institute of Biochemistry, University of Florence, Florence, Italy

关键词: Fructose 2;    6-bisphosphate;    Isoprenaline;    Prostacyclin;    Phorbol 12-myristate 13-acetate;    Thrombin;    Bradykinin;    (Human fibroblast);    Fru-2;    6-P2;    fructose 2;    6-bisphosphate;    PFK-1;    6-phosphofructo-1-kinase (ATP:fructose-6-phosphate 1-phosphotransferase;    EC 2.7.1.11);    PMA;    phorbol 12-myristate 13-acetate;    PGI2;    prostacyclin;    IBMX;    3-isobutyl-1-methylxanthine;   

DOI  :  10.1016/0014-5793(87)80185-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fructose 2,6-bisphosphate, the most potent activator of 6-phosphofructo-1-kinase, has been demonstrated to mediate the increase of glycolytic flux induced by mitogens human fibroblasts. In the present work the molecular basis of transmembrane control of fructose 2,6-bisphosphate has been investigated. Prostacyclin and isoprenaline, known to activate adenylate cyclase, are able to increase fructose 2,6-bisphosphate levels, indicating that in human fibroblasts cyclic AMP plays a positive role in the control of the metabolite concentration, opposite to that exerted in hepatocytes. Substances known to activate protein kinase C such as phorbol 12-myristate 13-acetate, or to stimulate phosphoinositide turnover such as thrombin and bradykinin are also effective in raising fructose 2,6-bisphosphate. Therefore, we conclude that cyclic AMP and protein kinase C are likely involved in the control of fructose 2,6-bisphosphate levels in human fibroblasts.

【 授权许可】

Unknown