A new carbene based heterobifunctional reagent Photochemical crosslinking of aldolase

FEBS Letters

Anjaneyulu, P.S.R.¹  Lala, A.K.¹  Mogre, R.M.¹  Batliwala, H.F.¹ 

[1] Biosciences and Engineering Group, Department of Chemistry, Indian Institute of Technology Bombay Powai, Bombay 400 076, India

关键词: Photoactivable group;    Heterobifunctional reagent;    Carbene;    Diazofluorene;    Aldolase crosslinking;   

DOI  :  10.1016/0014-5793(87)80965-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthesis of a new photoactivatable heterobifunctional crosslinking reagent, the N-oxysuccinimide ester of 2-carboxy-9-diazofluorene, is described. The ability of the parent chromophore 2-carbomethoxy-9-diazofluorene to insert into cyclohexane and methanol has been established. The reagent has been linked to aldolase and the stoichiometry determined. Photolysis of the probe-linked aldolase indicated that photolysis was very rapid and that the photolysed product was constituted of crosslinked dimer, trimer and tetramer. Increase in concentration of probe linked to aldolase followed by photolysis gave rise to largely tetramer and higher oligomers of aldolase. The use of this carbene-based reagent vis a vis arylazide-based reagent for studying protein crosslinking is discussed.

【 授权许可】

Unknown