Age‐dependent deamidation of chicken αA‐crystallin

FEBS Letters

Voorter, Christina E.M.¹  Bloemendal, Hans¹  de Jong, Wilfried W.¹  Roersma, Eric S.¹ 

[1] Department of Biochemistry, Center for Eye Research, University of Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands

关键词: Crystallin;    Deamidation;    Age dependence;    (Eye lens);   

DOI  :  10.1016/0014-5793(87)80935-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The major posttranslational modification product of αA-crystallin from chicken eye lenses has one more negative charge than the corresponding primary gene product. These polypeptides were compared by peptide mapping after tryptic digestion and cyanogen bromide cleavage, and the charge difference could be located in a peptide, comprising residues 146–150 of the amino acid sequence of αA-crystallin. Subsequent enzymatic hydrolysis with aminopeptidase showed that asparagine at position 149 of the primary gene product is replaced by aspartic acid. Two-dimensional gel electrophoresis of total lens homogenates from chickens of different ages revealed an age-dependent increase of the deamidated αA-subunit.

【 授权许可】

Unknown