Identification of a UDP‐Gal:β‐Galactoside βl→3‐galactosyltransferase in the albumen gland of the snail Lymnaea stagnalis

FEBS Letters

Joziasse, David H.²  Damen, Hanny C.M.²  Van den Eijnden, Dirk H.²  de Jong-Brink, Marijke¹  Edzes, Hommo T.² 

[1] Biology and Physical and Theoretical Chemistry, Vrije Universiteit, PO Box 7161, NL-1007 MC Amsterdam, The Netherlands;Departments of Medical Chemistry, Vrije Universiteit, PO Box 7161, NL-1007 MC Amsterdam, The Netherlands

关键词: Galactogen;    βl→3-Galactosyltransferase;    (Lymnaea stagnalis);    OSM;    ovine submaxillary mucin;    PSM;    porcine submaxillary mucin;    HPLC;    high-pressure liquid chromatography;    GC/MS;    gas chromatography/mass spectrometry;    cer;    ceramide;    pNph;    p-nitrophenol;    GalNAc;    N-acetylgalactosamine;    Fuc;    L-fucose (all sugars are of the D configuration unless noted otherwise);   

DOI  :  10.1016/0014-5793(87)80368-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Detergent extracts of the albumen gland of the snail Lymnaea stagnalis contain an enzyme activity that transfers Gal from UDP-Gal to acceptor substrates with terminal non-reducing β-galactose residues. The products formed with lactose (Galβl→4Glc) as the acceptor were characterized by HPLC, and subjected to 400-MHz ¹H-NMR and methylation analysis. The main product was shown to have the structure Gal-βl→3Galβl→4Glc. Therefore, the galactosyltransferase can be identified as a UDP-Gal:β-galactoside βl→3-galactosyltransferase. In view of its linkage and acceptor specificity, the enzyme may be essential to the biosynthesis of galactogen, the main polysaccharide produced by albumen glands of L. stagnalis.

【 授权许可】

Unknown