Crystallization of 70 S ribosomes and 30 S ribosomal subunits from Thermus thermophilus

FEBS Letters

Shirokov, V.A.²  Trakhanov, S.D.¹  Agalarov, S.C.²  Garber, M.B.²  Yusupov, M.M.²  Tischenko, S.V.²  Ryazantsev, S.N.² 

[1] Institute of Crystallography, Academy of Sciences of the USSR, Moscow, USSR;Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

关键词: Ribosome;    30 S Ribosomal subunit;    Three-dimensional crystal;    Electron microscopy;    (Thermus thermophilus);   

DOI  :  10.1016/0014-5793(87)80838-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Well-ordered three-dimensional crystals of 70 S ribosomes and 30 S ribosomal subunits from extremely thermophilic bacteria Thermus thermophilus have been obtained. Positively stained thin sections of the crystals have been analyzed by electron microscopy. Redissolved crystalline ribosomes and small ribosomal subunits reveal sedimentation constants of 70 S and 30 S, respectively, and are functionally active in the poly(U)-system.

【 授权许可】

Unknown