Clathrin β‐light chain of rat liver coated vesicles is phosphorylated in vitro and in vivo

FEBS Letters

Loeb, Jacques¹  Cantournet, Benoit¹  Creuzet, Claudine¹  Komano, Odile¹ 

[1] Laboratoire de Biochimie des Régulations, IRSC, BP No. 8, 94802 Villejuif Cedex, France

关键词: Coated vesicle;    Poly(L-lysine);    Clathrin β-light chain;    Casein kinase II;    Protein phosphorylation;    Mes;    (2-(N-morpholino)ethanesulfonic acid;    PMSF;    phenylmethylsulfonylfluoride;    PAGE;    polyacrylamide gel electrophoresis;    PNPP;    p-nitrophenyl phosphate;    disodium salt;    NEM;    N-ethylmaleimide;    PCA;    perchloric acid;   

DOI  :  10.1016/0014-5793(87)80892-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Clathrin β-light chain of rat liver coated vesicles is phosphorylated in vitro in the presence of poly(L-lysine) by an endogenous protein kinase which appears to be similar to casein kinase II. Clathrin β-light chain is also phosphorylated in vivo. After injection of [³²P]phosphate into rats and preparation of purified coated vesicles in the presence of phosphatase inhibitors, electrophoretic analysis showed the presence of several labeled polypeptides including clathrin β-light chain. A polypeptide of 50 kDa, which may correspond to the major polypeptide phosphorylated in vitro of coated vesicles, is also labeled in vivo.

【 授权许可】

Unknown