Effect of DFP on loading of fura 2/AM and quin 2/AM into single smooth muscle cells prepared from guinea pig taenia coli

FEBS Letters

Ohtsuka, Kazuko¹  Maruyama, Ikuo¹  Oyamada, Hideto¹  Hasegawa, Tomohiko¹  Momose, Kazutaka¹ 

[1] Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Hatanodai 1-5-8, Shinagawa-ku, Tokyo 142, Japan

关键词: Quin 2;    Fura 2;    Diisopropyl fluorophosphate;    (Single smooth muscle cell;    Guinea pig taenia coli);   

DOI  :  10.1016/0014-5793(87)80881-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of diisopropyl fluorophosphate (DFP), a potent cholinesterase (ChE) inhibitor, on loading quin 2 acetoxymethyl ester (quin 2/AM) and fura 2/AM into smooth muscle cells isolated from guinea pig taenia coli was investigated spectrofluorometrically. The presence of DFP during the loading permitted the incorporation of quin 2 into the cells, so that it became possible to measure intracellular Ca²⁺ concentrations using the ester of this dye. Also, DFP significantly enhanced the incorporation of fura 2 into the cells. These results indicate that loading of quin 2/AM and fura 2/AM into the smooth muscle cells may depend on the suppression of ChE or various serine protease activities outside cells.

【 授权许可】

Unknown