Benzodiazepine agonists protect a histidine residue from modification by diethyl pyrocarbonate whereas propyl β‐carboline does not

FEBS Letters

Rossier, J.¹  Lambolez, B.¹ 

[1] Laboratoire de Physiologie Nerveuse CNRS, 91190 Gif-sur-Yvette, France

关键词: Benzodiazepine;    β-Carboline;    Binding site;    pH sensitivity;    Diethyl pyrocarbonate;    Histidine residue;    DEP;    diethyl pyrocarbonate;    Bdz;    benzodiazepine;    B-CCP;    propyl β-carboline-3-carboxylate;    B-CCM;    methyl β-carboline-3-carboxylate;    Flu;    flunitrazepam;    GABA;    gamma-amino butyric acid;   

DOI  :  10.1016/0014-5793(87)80240-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The pH sensitivity of benzodiazepine binding suggests that a histidine residue may be present in, or close to the benzodiazepine binding site. This was confirmed by the selective modification of histidine residues using diethyl pyrocarbonate which was found to block both benzodiazepine and β-carboline binding. In order to assess whether this histidine residue is located in or adjacent to the benzodiazepine and β-carboline binding sites, experiments were performed using either benzodiazepine or β-carboline to protect against diethyl pyrocarbonate treatment. It was found that benzodiazepine agonists, but not propyl β-carboline protect the benzodiazepine binding sites from diethyl pyrocarbonate modification.

【 授权许可】

Unknown