Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [3H]dihydrorotenone

FEBS Letters

Attardi, G.¹  Earley, Fergus G.P.²  Patel, Salil D.²  Ragan, C.Ian² 

[1]Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA

[2]Department of Biochemistry, University of Southampton, Southampton, SO9 3TU, England

关键词: NADH dehydrogenase;    Mitochondrial genome;    Rotenone;    ND-1 gene product;   

DOI  :  10.1016/0014-5793(87)81200-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [³H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of M _r 33 000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C-terminal sequence of the human ND-1 gene product.

【 授权许可】

Unknown