Cause of the blue shift of the absorption spectrum of tetranitromethane‐treated bacteriorhodopsin

FEBS Letters

Tokunaga, Fumio¹  Higashiyama, Hiroyuki¹ 

[1] Department of Physics, Faculty of Science, Tohoku University, Aobayama, Sendai 980, Japan

关键词: Bacteriorhodopsin;    Purple membrane;    Retinal;    Tetranitromethane;    Nitration;    Reconstitution;    BR;    bacteriorhodopsin;    PM;    purple membrane;    BO;    bacterioopsin;    TNM;    tetranitromethane;    TNM-BR;    TNM-treated BR;    TNM-BO;    TNM-treated BO;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(87)81063-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The reaction of tetranitromethane with bacteriorhodopsin under irradiation was separated into two stages by selecting wavelengths of the irradiation light. The first stage of the reaction was characterized by a blue shift of the absorption spectrum and the second stage was characterized by decrease of the absorption of the visible region. The blue shift at the first stage was not caused by nitration of tyrosine residues but a modification of the chromophore. This conclusion was confirmed by reconstitution experiments and chromophore analysis. Nitration of tyrosine residues progressed at the second stage of the reaction.

【 授权许可】

Unknown