FEBS Letters | |
A fast transient hyperpolarization occurs during the sea urchin sperm acrosome reaction induced by egg jelly | |
Darszon, A.2  González-Martínez, M.1  | |
[1]Departments of Pharmacology, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14-740,07000 México City DF, Mexico | |
[2]Departments of Pharmacology, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14-740, 07000 México City DF, Mexico | |
关键词: Hyperpolarization; Acrosome reaction; Fluorescence; (Sea urchin sperm); ASW; artificial seawater; pHi; intracellular pH; diS-C3-(5); 3; 3′-dipropylthiadicarbocyanine iodide; [K+]ext; external K+ concentration; Vm; plasma membrane potential; | |
DOI : 10.1016/0014-5793(87)81055-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The egg jelly-induced sea urchin sperm depolarization, assayed with the membrane potential-sensitive dye diS-C3-(5), is preceded by a fast quenching, which was initially taken as an artifact. Here we show that part of this quenching results from a K+ -dependent transient hyperpolarization (∼4 s). Seawater containing 25–35 mM KCl inhibits this hyperpolarization, the depolarization and the acrosome reaction induced by egg jelly, or by the acrosome reaction inducing factor purified therefrom. These results suggest that egg jelly induces a transient hyperpolarization mediated by an increase in K+ permeability, which may be involved in triggering the acrosome reaction
【 授权许可】
Unknown
【 预 览 】
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