Formation of lipoxin A by granulocytes from eosinophilic donors

FEBS Letters

Samuelsson, Bengt¹  Palmblad, Jan¹  Hirsch, Ulf¹  Serhan, Charles N.¹ 

[1] Department of Physiological Chemistry and Department of Medicine 3 at Soderjukhuset, Karolinska Institute, S-104 01 Stockholm, Sweden

关键词: Lipoxygenase product;    Eosinophil;    Arachidonic acid;    Leukocyte;    (Human);    RP-HPLC;    reverse-phase high-pressure liquid chromatography;    GC;    gas-liquid chromatography;    MS;    mass spectrometry;    Me3Si;    trimethylsilyl;    LX;    lipoxin;    lipoxin A (LXA);    5(S);    6(R);    15(S) -trihydroxy-7;    9;    13-trans-11-cis-eicosatetraenoic acid;    6(S)-LXA;    5(S);    6(S);    15(S)-trihydroxy-7;    9;    13-trans-11 -cis-eicosatetraenoic acid;    lipoxin B (LXB);    5(S);    14(R);    15(S) -trihydroxy-6;    10;    12-trans-8-cis-eicosatetraenoic acid;    15 -HETE;    15(S)-hydroxy-5;    8;    11-cis-13-trans -eicosatetraenoic acid;    HES;    hypereosinophilic syndrome;    5;    15 -DHETE;    5(S);    15(S)-dihydroxy-6;    13-trans-8;    11-cis -eicosatetraenoic acid;   

DOI  :  10.1016/0014-5793(87)80671-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The formation of arachidonic acid-derived lipoxygenase products was examined with human granulocytes obtained from eosinophilic donors. These eosinophil-enriched leukocyte populations, challenged in vitro with the ionophore of divalent cations A23187, transformed both exogenous and endogenous sources of arachidonic acid to several lipoxygenase-derived products, including 5(S),6(R),15(S)-trihydroxy-7,9, 13-trans-11-cis-eicosatetraenoic acid (lipoxin A). Lipoxin A was detected and characterized by high-pressure liquid chromatography (HPLC), ultraviolet absorbance, and gas-liquid chromatography-mass spectroscopy. Neither lipoxin B nor 6(S)-LXA was consistently detected in extracts from these incubations. The amounts of lipoxin A formed were proportional to the percentage of eosinophils present in the suspension. The results indicate that granulocytes from eosinophilic donors can generate lipoxin A.

【 授权许可】

Unknown