Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C

FEBS Letters

Lundwall, Åke¹  Abrahamson, Magnus¹  Grubb, Anders¹  Olafsson, Isleifur¹ 

[1] Department of Clinical Chemistry, University of Lund, Malmö General Hospital, S-214 01 Malmö, Sweden

关键词: Cerebral hemorrhage;    Cystatin C;    Cysteine proteinase;    Enzyme inhibitor;    cDNA cloning;    Nucleotide sequence;   

DOI  :  10.1016/0014-5793(87)80695-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recombinant cystatin C producing clones were isolated from a human placenta λgt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.

【 授权许可】

Unknown