Modified forms of vasopressin and oxytocin in a bovine pineal preparation

FEBS Letters

Noteborn, H.P.J.M.²  Ebels, I.²  Burbach, J.P.H.¹ 

[1] Rudolf Magnus Institute for Pharmacology, Medical Faculty, Utrecht University, Vondellaan 6, NL-3521 GD Utrecht, The Netherlands;>Department of Organic Chemistry, Transitorium III, Utrecht University, Padualaan 8, NL-3508 TB Utrecht The Netherlands

关键词: Peptide isolation;    Neuropeptide;    Oxytocin;    Vasopressin;    Post-translational modification;    (Bovine pineal);   

DOI  :  10.1016/0014-5793(87)80689-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A bovine pineal acid extract displays a vasotocin-like bioactivity in several bioassays, and is recognized by antibodies against the Pro-Arg-Gly-amide ending common to vasopressin and vasotocin. By using molecular sieve filtration and reversed-phase HPLC, a vasopressin- and oxytocin-like peptide was isolated from this pineal preparation, while no evidence for a vasotocin-like peptide was obtained. The isolated neuropeptides contain a modified amino acid at position 2. This structural difference with authentic pituitary vasopressin and oxytocin may alter their biological and immunological properties, which have been interpreted as vasotocin-like, and thus underlies the controversy concerning the existence of vasotocin in the mammalian pineal gland.

【 授权许可】

Unknown