The binding of glucosylceramidase to glucosylceramide is promoted by its activator protein

FEBS Letters

Muscillo, M.¹  Suzuki, K.²  Salvioli, R.¹  Tatti, M.¹  Gallozzi, E.¹  Vaccaro, A.M.¹ 

[1] >Department of Metabolism and Pathological Biochemistry, Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, Italy;Biological Sciences Research Center, Department of Neurology and Psychiatry, University of North Carolina School of Medicine, Chapel Hill, NC, USA

关键词: Protein activator;    Glucosylceramidase;    Gaucher's disease;   

DOI  :  10.1016/0014-5793(87)80687-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protein activator of glucosylceramidase (EC 3.2.1.45) has been previously identified by us in human placenta [(1985) Biochim. Biophys. Acta 836, 157–166]. In the present paper we report that its function in vitro is to stimulate the binding of the enzyme to its substrate, glucosylceramide. After the purification step which frees the enzyme of most of its activator protein (octyl-Sepharose 4B chromatography), the capacity of glucosylceramidase to bind to the glucosylceramide micelles is dramatically decreased. The addition of the activator protein to the purified enzyme restores this binding.

【 授权许可】

Unknown