Counterion collapse and the effect of diamines on bacteriorhodopsin

FEBS Letters

Marinetti, Tim¹ 

[1] Rockefeller University, 1230 York Avenue, New York NY 10021, USA

关键词: Bacteriorhodopsin;    Purple membrane;    Proton pump;    Counterion;    Surface charge;    Diamine;   

DOI  :  10.1016/0014-5793(87)80775-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A recent report of electrical measurements on oriented bacteriorhodopsin in gels [(1986) FEBS Lett. 195, 164–168] concluded that low concentrations of diamines reversed the direction of the proton pump. Calculations are presented which show that in low diamine concentrations, charge displacements of the counterion atmosphere in the direction opposite to proton pumping are expected following H⁺ ejection. It is also shown that the effect will be sharply reduced by raising the diamine concentration or by adding excess salt, as was observed. Hence it is not necessary to conclude that diamines reverse the direction of the proton pump itself.

【 授权许可】

Unknown