The kinetics of calcium binding to fura‐2 and indo‐1

FEBS Letters

Jackson, A.P.¹  Ashley, C.C.²  Timmerman, M.P.²  Bagshaw, C.R.¹ 

[1] Department of Biochemistry, Leicester University, Leicester LE1 7RH USA;University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, England

关键词: Fura-2;    Indo-1;    Ca2+;    Mg2+;    Stopped-flow spectrometry;    Fluorescence;   

DOI  :  10.1016/0014-5793(87)80752-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinetics of Ca²⁺ dissociation from fura-2 and indo-1 were measured using a stopped-flow spectrofluorimeter. The dissociation rate constants were 84 s⁻¹ and 130 s⁻¹, respectively, in 0.1 M KCl at 20°C. The rate constants were insensitive to pH over the range 7.0 to 8.0. The second order association rate constants were estimated indirectly to be in the region of 5 × 10⁸ M⁻¹·s⁻¹ and thus approach the diffusion-controlled limit. The results demonstrate that these new generation indicators are well-suited to measure rapid changes in concentration of intracellular Ca²⁺.

【 授权许可】

Unknown