Association of calpains 1 and 2 with protein kinase C activities

FEBS Letters

Pallet, Véronique¹  Savart, Michel¹  Belamri, Mohammed¹  Ducastaing, André¹ 

[1] Laboratoire de Biochimie et Technologie des Aliments, Département Alimentation et Nutrition, Université de Bordeaux I, Avenue des Facultés, 33405 Talence Cédex, France

关键词: Calpain 1;    Calpain 2;    Protein kinase C;   

DOI  :  10.1016/0014-5793(87)80749-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calpains 1 and 2 co-eluted with protein kinase C activities after hydrophobic (phenyl-Sepharose) and anion-exchange (Mono Q) chromatographies of a 100 000 × g supernatant which was defined as cytosol. After centrifugation of the cytosol at 200 000 × g for 16 h, the major part of calpain 1 and of its associated protein kinase C activity was recovered in the pellet, when the major part of calpain 2, also associated to a protein kinase C activity, was present in the resulting supernatant. Polyacrylamide gel electrophoresis of the fractions eluted from the Mono Q column, which contained calpains 1 or 2 and their associated protein kinase C activities, revealed two main bands with a molecular mass of 80 and 28 kDa.

【 授权许可】

Unknown