In vivo enzymology: 13C NMR measurement of a kinetic isotope effect for methanol oxidation in Methylosinus trichosporium OB3b

FEBS Letters

Mason, Ralph P.²  Cornish, Alex¹  Sanders, Jeremy K.M.² 

[1] Biotechnology Centre, Cranfield Institute of Technology, Cranfield, Bedford, England;University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW England

关键词: Enzyme kinetics;    NMR;    Methanol oxidation;    Isotope effect;    Formaldehyde production;   

DOI  :  10.1016/0014-5793(87)80745-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The competitive oxidation of ¹³CH₃OH and ¹³CD₃OH has been observed using in vivo ¹³C NMR spectroscopy. Simultaneous ¹H and ²H decoupling gave isotopically shifted ¹³C singlets for the two methanol isotopomers. The measured enzymic isotope effect, kH/kD is approx. 1.8, indicating that CH bond cleavage is not rate-determining.

【 授权许可】

Unknown