Mucin‐carbohydrate directed monoclonal antibody

FEBS Letters

Fukui, Shigeyuki¹  Numata, Yoshito¹  Funakoshi, Ikuo¹  Kawasaki, Toshisuke¹  Kitagawa, Hiroshi¹  Kurosaka, Akira¹  Yamashina, Ikuo¹  Nakada, Hiroshi¹ 

[1] Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606, Japan

关键词: Monoclonal antibody;    Colonic cancer;    Cancer-associated carbohydrate antigen;    Mucin;   

DOI  :  10.1016/0014-5793(87)80128-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To raise monoclonal antibodies recognizing cancer-associated alterations of the carbohydrate structure of glycoproteins, Balb/c mice were immunized with human colonic cancer cells (LS 180 from ATCC). One of the generated hybridomas produced a monoclonal antibody that bound to the carbohydrate moiety of mucin-type glycoproteins from LS 180. The antibody did not bind to glycoproteins from another colonic cancer cell line, SW 1116, or to glycolipids from any of the colonic cancer cell lines. The antibody bound to ovine and bovine submaxillary mucins (OSM and BSM). NeuAcα2→6Ga1NAc seemed to be involved in the epitope.

【 授权许可】

Unknown