Isolation and characterization of a rubredoxin and a flavodoxin from Desulfovibrio desulfuricans Berre‐Eau

FEBS Letters

Fauque, Guy D.³  LeGall, Jean³  Moura, José J.G.¹  Xavier, António V.¹  Galliano, Nicole²  Moura, Isabel¹ 

[1] Centro de Quimica Estrutural, Complexo I, UNL, Av. Rovisco Pais, 1000 Lisboa, Portugal;ARBS, ECE, CEN Cadarache, 13108 Saint-Paul-lez-Durance Cédex, France;Department of Biochemistry, University of Georgia, School of Chemical Sciences, Athens, GA 30602, USA

关键词: Rubredoxin;    Flavodoxin;    Electron transfer;    Sulfate reduction;    (Desulfovibrio);    FMN;    flavin adenine mononucleotide;    HPLC;    high-pressure liquid chromatography;    PITC;    phenylisothiocyanate;    ϵM;    molar extinction coefficient;   

DOI  :  10.1016/0014-5793(87)80114-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A rubredoxin and a flavodoxin have been purified and characterized from soluble extracts of a sulfate-reducing bacterium able to grow with N₂ as the only nitrogen source: Desulfovibrio desulfuricans strain Berre-Eau. These two electron carriers have characteristics similar to homologous proteins found in other Desulfovibrio species (molecular mass, absorption spectrum, extinction coefficient and amino acid composition). In contrast to rubredoxin, flavodoxin mediates electron transfer in the reduction of sulfite to sulfide and in hydrogen evolution from pyruvate, when in the presence of hydrogenase

【 授权许可】

Unknown