Potato virus X‐related single‐ and double‐stranded RNAs

FEBS Letters

Grama, Dmitrii P.¹  Morozov, Sergei Yu.²  Atabekov, Joseph G.²  Dolja, Valerian V.² 

[1] D.K. Zabolotnyi Institute of Microbiology and Virology, Kiev 252142 USSR;Department of Virology, Moscow State University, Moscow 119899 USSR

关键词: RNA;    Potato virus X;    subgenomic RNA;    double-stranded RNA;    (Plant virus);   

DOI  :  10.1016/0014-5793(87)80076-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Six species of 3′-coterminal poly(A) -containing RNAs of subgenomic (sg) size have been found in plants infected with potato virus X (PVX): two major (0.9 kb — the coat protein mRNA, and 2.1 kb) and four minor (1.4, 1.8, 3.0 and 3.6 kb). The 5′-end of the shortest sgRNA is located 26 nucleotides upstream of the initiating codon of the coat protein gene (812 nucleotides from the 3′-terminal poly(A) tract of the PVX genomic RNA). Double-stranded analogues have been found for most sgRNAs. The genomic-size double-stranded RNA (the replicative form) is shown to carry a poly(A)-poly(U) hybrid of a predominant length of 150–250 bp on one end, and an unpaired G residue on the other (the 3′-end of the negative chain). In contrast to this(—) the chains of double-stranded 0.9 and 2.1 kbp sgRNAs lack the unpaired G and both end in C.

【 授权许可】

Unknown