ESR signals from stimulated and resting porcine blood neutrophils

FEBS Letters

Hata-Tanaka, Akiko¹  Kakinuma, Katsuko²  Chiba, Tsukasa² 

[1] The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, 3-18, Tokyo 113, Japan

关键词: Neutrophil;    NADPH oxidase;    Superoxide generating enzyme;    Cytochrome b-558;    ESR signal;   

DOI  :  10.1016/0014-5793(87)80070-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The NADPH oxidase in neutrophils was specifically solubilized from membrane vesicles of porcine blood neutrophils and rapidly concentrated by immunoprecipitation with cross-reacting anti-P-450 reductase IgG. The precipitates from both myristic acid-stimulated and resting cells contained one third of the cytochrome b-558 and were slightly contaminated with myeloperoxidase. The immunoprecipitate from stimulated cells gave rhombic high-spin ESR signals of a heme at g = 6.47 and 5.49, which were insensitive to KCN, whereas the preparation from resting cells did not give these signals. The rhombic high-spin signals are discussed in view of the participation of cytochrome b-558 in the NADPH oxidase system.

【 授权许可】

Unknown