FEBS Letters | |
De novo CDP‐choline‐dependent glycerophosphorylcholine synthesis and its involvement as an intermediate in phosphatidylcholine synthesis | |
Infante, J.P.1  | |
[1] Division of Nutritional Sciences, Martha Van Rensselaer Hall, Cornell University, Ithaca, NY 14853, USA | |
关键词: Glycerophosphorylcholine; Phosphatidylcholine; CDP-choline; Phosphatidylethanolamine; (Muscle); PC; phosphatidylcholine; PE; phosphatidylethanolamine; PG; phosphatidylglycerol; PI; phosphatidylinositol; PS; phosphatidylserine; G3P; sn-glycerol-3-phosphate; GPC; sn-glycerol-3-phosphorylcholine; C22:6n—3; all-cis-4; 7; 10; 13; 16; 19-docosahexaenoate; | |
DOI : 10.1016/0014-5793(87)80031-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The activity of CDP-choline-dependent glycerophosphorylcholine synthetase (CDP-choline:sn-3-glycerophosphate cholinetransferase), a newly discovered enzyme involved in the recently proposed pathways of acyl-specific phosphatidylcholine synthesis, is reported in rat liver. Endogenous CDP-choline, synthesized via the CMP-driven back reaction of phosphorylcholine transferase, is also shown to be a choline donor for this glycerophosphorylcholine synthetase. The function of glycerophosphorylcholine as an intermediate in phosphatidylcholine synthesis is demonstrated by specific isotope trapping whereby unlabelled glycerophosphorylcholine inhibited label incorporation from sn-[14C]glycerol-3-phosphate into phosphatidylcholine in mouse gastrocnemius, a tissue that is essentially devoid of the cytidine pathway for phosphatidylcholine synthesis and uses a non-allelic glycerophosphorylcholine synthetase (exogenous PC:sn-3-glycerophosphate cholinetransferase) in the synthesis of glycerophosphorylcholine.
【 授权许可】
Unknown
【 预 览 】
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