Interaction of chlorophyll a′ with the 65 kDa subunit protein of photosystem I reaction center

FEBS Letters

Kobayashi, Masami¹  Hiyama, Tetsuo³  Watanabe, Tadashi¹  Nakazato, Masataka² 

[1] Institute of Industrial Science, University of Tokyo, Roppongi, Minato-ku, Tokyo 106 Japan;Nampo Pharmaceutical Co. Ltd, Nihonbashi-honcho, Chuo-ku, Tokyo 103, Japan;Department of Biochemistry, Saitama University, Urawa 338, Japan

关键词: Chlorophyll a′;    P700;    Photosystem I;    Photosynthesis;   

DOI  :  10.1016/0014-5793(87)80020-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 65 kDa polypeptide subunit depleted of P700 was prepared from a photosystem I reaction center preparation and mixed with chlorophyll a′ (C-10 epimer of chlorophyll a) to yield a complex exhibiting a tripleheaded spectrum with absorbance maxima at 673, 692 and 707 nm. The difference spectra (oxidized-minus-untreated and light-minus-dark) had a major trough at 707 nm and minor ones at 690 and 430 nm. The overall shape of the spectra resembled well that of P700 with a small red shift. A rapidly decaying flash-induced absorbance change was observed at 430 nm with a half decay time of less than 500 μs in a preparation supplemented with an electron donor system.

【 授权许可】

Unknown