Competition between tetracycline and tRNA at both P and A sites of the ribosome of Escherichia coli

FEBS Letters

Epe, Bernd²  Hornig, Horst³  Woolley, Paul¹ 

[1] Kemisk Institut, Aarhus Universitet, 8000 Århus C, Denmark;Institut für Toxikologie, Versbacherstrasse 9, 8700 Würzburg, FRG;M'ax- Planck-Institut für molekulare Genetik (Abt. Wittmann), Ihnestrasse 63, D-1000 Berlin 33, Germany

关键词: Fluorescence anisotropy;    Tetracycline;    Ribosome;    A site;    P site;    Antibiotic;   

DOI  :  10.1016/0014-5793(87)81539-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fluorescence anisotropy studies performed on 6-demethylchlortetracycline, binding to the ribosome of E. coli in competition with tRNA at the P site or at both P and A sites, have provided a quantitative assessment in situ of the interaction of this antibiotic with the A site and have demonstrated that there is also an interaction between tetracycline and the P site.

【 授权许可】

Unknown