Reannealing of phalloidin‐treated actin filaments during recovery after sonication and its inhibition by β‐actinin

FEBS Letters

Oosawa, Masatake¹  Maruyama, Koscak¹ 

[1] Department of Biology, Faculty of Science, Chiba University, Chiba 260, Japan

关键词: Actin;    Phalloidin;    Reannealing;    Actin filament;    β-Actinin;   

DOI  :  10.1016/0014-5793(87)81537-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phalloidin (2 mol per mol actin)-treated pyrenyl F-actin showed a critical concentration of 1.8 μM in the presence of 10 mM KCl, 0.2 mM ADP, and 5 mM Tris-HCl buffer, pH 8.0 at 25°C. The filament weight concentration did not change at all during and after sonication, yet degrees of flow birefringence increased and the filament number concentration decreased after the termination of sonication. The latter changes were not affected by EDTA, but inhibited by β-actinin. These observations suggest that reannealing of short pieces of phalloidin-treated actin filaments fragmented during sonication takes place during recovery after sonication.

【 授权许可】

Unknown