Evidence for bile acid glucosides as normal constituents in human urine

FEBS Letters

Egestad, B.²  Sjövall, J.²  Matern, H.¹  Marschall, H.-U.²  Matern, S.¹ 

[1] Department of Internal Medicine III, Klinikum der RWTH, Pauwelsstr., D-5100 Aachen, FRG;Department of Physiological Chemistry, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden

关键词: Bile acid;    Glucoside;    Microsome;    (Human liver;    Urine);   

DOI  :  10.1016/0014-5793(87)81532-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A glucosyltransferase catalysing formation of bile acid glucosides was recently isolated from human liver microsomes. In order to investigate the potential occurrence of such bile acid derivatives in vivo, a method was devised for their isolation and purification from urine. Conditions were established with the aid of glucosides of radiolabelled, unconjugated glycine and taurine conjugated bile acids prepared enzymatically using human liver microsomes. Analysis by gas chromatography and mass spectrometry of methyl ester trimethylsilyl ether derivatives indicated the excretion of glucosides of nonamidated hyodeoxycholic, chenodeoxycholic, deoxycholic, ursodeoxycholic and cholic acids and of glycine and taurine conjugated chenodeoxycholic and cholic acids. Additional compounds were present giving mass spectral fragmentation patterns typical ofdi- and trihydroxy bile acid glycosides. Semiquantitative estimates indicated a total daily excretion of about 1 μmol.

【 授权许可】

Unknown