Oxidation‐reduction midpoint potentials of the molybdenum center in spinach NADH:nitrate reductase

FEBS Letters

Solomonson, Larry P.²  Barber, Michael J.²  Notton, Brian A.¹ 

[1]University of Bristol, Long Ashton Research Station, Long Ashton, Bristol BS18 9AF, England

[2]Department of Biochemistry, University of South Florida, College of Medicine, Tampa, FL 33612, USA

关键词: Nitrate reductase;    Molybdenum;    Redox potential;    (Spinacia oleracea);    Mops;    3-(N-morpholino)propanesulfonic acid;    MV.+;    methyl viologen radical cation;   

DOI  :  10.1016/0014-5793(87)81524-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidation-reduction midpoint potentials for the molybdenum center in assimilatory NADH:nitrate reductase isolated from spinach (Spinacia oleracea) have been determined at pH 7.0 in the presence of dye mediators using EPR spectroscopy to monitor formation of Mo(V). Values for the Mo(VI)/Mo(V) and Mo(V)/Mo(IV) couples were determined to be −8 and −42 mV, respectively.

【 授权许可】

Unknown