dam methylase from E. coli

FEBS Letters

Guschlbauer, Wilhelm¹  Kriebardis, Anastasios¹ 

[1] Service de Biochimie, Bat. 142, Département de Biologie, Centre d'Etudes Nucléaires de Saclay, F-91191 Gif-sur-Yvette Cédex, France

关键词: Methylation;    α-Helix;    β-Sheet;    Secondary structure;    Predictive method;   

DOI  :  10.1016/0014-5793(87)81509-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The enzyme dam methylase which recognizes and methylates the adenine in the palindromic sequence GATC in DNA was isolated and the secondary structure was determined by CD spectroscopy and various predicting methods from the amino acid sequence. The interaction of dam methylase with S-adenosylmethionine was studied by CD spectroscopy indicating a decrease of the percentage of α-helix as the amount of S-adenosylmethionine bound to the enzyme was increased.

【 授权许可】

Unknown