Demonstration of an epidermal growth factor‐dependent 58 kDa phosphoprotein secreted by rat kidney fibroblasts

FEBS Letters

Binas, Bert¹  Grosse, Richard¹ 

[1] Central Institute of Molecular Biology of the Academy of Sciences of the German Democratic Republic, Robert-Rössle-Straße10, 1115 Berlin-Buch, GDR

关键词: Epidermal growth factor;    Phosphoprotein secretion;    (Normal rat kidney fibroblast);    NRK;    normal rat kidney fibroblasts (clone 49F);    EGF;    epidermal growth factor;    PDGF;    platelet-derived growth factor;    FGF;    fibroblast growth factor;    TGF-β;    transforming growth factor-β;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/0014-5793(87)81484-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Epidermal growth factor and 12-O-tetradecanoylphorbol-13-acetate increased the amount of ³²P_i found as phosphoserine in a major, hitherto not described 58 kDa phosphoprotein (pp58) secreted by normal rat kidney fibroblasts. Platelet-derived growth factor, insulin, nerve growth factor and fibroblast growth factor did not affect pp58 while transforming growth factor β decreased the accumulation of radioactivity into pp58. Cycloheximide, actinomycin D and ammonium chloride suppressed the labelling of pp58.

【 授权许可】

Unknown