Regulatory role of the ATPase inhibitor protein on proton conduction by mitochondrial H+‐ATPase complex

FEBS Letters

Zanotti, Franco¹  Scarfò, Rosanna¹  Che, Yan Wu¹  Guerrieri, Ferruccio¹  Papa, Sergio¹ 

[1] Institute of Medical Biochemistry and Chemistry and Centre for the Study of Mitochondria and Energy Metabolism, CNR, University of Bari, Bari, Italy

关键词: H+-ATPase;    Inhibitor protein;    Proton conductivity;    Fo;    membrane integral sector of the mitochondrial membrane;    F1;    catalytic part of mitochondrial H+-ATPase;    IF1;    natural inhibitor protein of mitochondrial H+-ATPase;    ESMP;    submitochondrial particles prepared in the presence of EDTA;    Sephadex-ESMP;    particles depleted of IF1 by passing ESMP through a Sephadex column;    USMP;    particles depleted of F1 by urea treatment of Sephadex-ESMP;   

DOI  :  10.1016/0014-5793(87)81466-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study shows that the natural inhibitor protein of mitochondrial H⁺-ATPase complex (IF₁) inhibits, in addition to the catalytic activity, the proton conductivity of the complex. The inhibition of ATPase activity by IF₁ is less effective in the purified F₁ than in submitochondrial particles where F₁ is bound to F₀. No inhibition of H⁺ conductivity by F₀ is observed in F₁-depleted particles

【 授权许可】

Unknown