Identification of disulfide bonds in carboxy‐terminal propeptides of human type I procollagen

FEBS Letters

Koivu, Juha¹ 

[1] Collagen Research Unit, University of Oulu, Department of Medical Biochemistry, SF-90220 Oulu, Finland

关键词: Type I procollagen;    Disulfide bond;    Carboxy-terminal propeptide;    DTT;    dithiothreitol;    CNBr;    cyanogen bromide;   

DOI  :  10.1016/0014-5793(87)81350-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intra-chain and inter-chain disulfide bonds within the carboxy-terminal propeptides of human type I procollagen were studied using cyanogen bromide cleavage of the propeptides and electrophoresis on SDS-polyacrylamide/glycerol gels. The results could provide a better understanding of the assembly of proα-chains into procollagen.

【 授权许可】

Unknown