NMR study of the interaction of P‐450 with 4‐methoxypyridine

FEBS Letters

Woldman, Ya.Yu.²  Lyakhovich, V.V.²  Weiner, L.M.¹  Gulayeva, L.F.² 

[1] Institute of Chemical Kinetics and Combustion, Novosibirsk 630090, USSR;Institute of Clinical and Experimental Medicine, Novosibirsk630091, USSR

关键词: Cytochrome P-450;    4-Methoxypyridine;    1H-NMR;    Enzyme-substrate interaction;   

DOI  :  10.1016/0014-5793(87)81555-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Longitudinal relaxation (T₁) measurements for all lines (α-CH, β-CH, O-CH₃) in the 4-methoxypyridine ¹H-NMR spectrum were used to study the interaction of 4-methoxypyridine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the observed T₁ ⁻¹ value was determined from its dependence on 4-methoxypyridine concentration. In the P-450-4-methoxypyridine complex the latter is oriented so that the nitrogen of pyridine is directed towards the Fe³⁺.

【 授权许可】

Unknown