Radioactively labeled ether lipids by biotransformation of symmetrical alkylglycerols in cell suspension cultures of rape

FEBS Letters

Weber, Nikolaus¹  Mangold, Helmut K.¹ 

[1] Bundesanstalt für Fettforschung, Institut für Biochemie und Technologie, H.P. Kaufmann-Institut, Piusallee, D-4400 Münster, FRG

关键词: Ether lipid;    Platelet-activating factor;    Lyso-PAF;    Cell culture;    PAF analog;    Lyso-PAF analog;   

DOI  :  10.1016/0014-5793(87)81441-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Biotransformation of 2-O-[1'-¹⁴C]hexadecylglycerol by photomixotrophic rape (Brassica napus) cells in suspension culture leads in appreciable yield (> 60%) to 1-acyl-2-O-[1'-¹⁴C]hexadecyl-sn-glycero-3-phosphocholines. Alkaline hydrolysis of the acyl moiety in these phospholipids yields 2-O-[1'-¹⁴C]hexadecyl-sn-glycero-3-phosphocholine which is acetylated to 1-acetyl-2-O-[1'-¹⁴C]hexadecyl-sn-glycero-3-phosphocholine, i.e. a positional isomer of platelet-activating factor.

【 授权许可】

Unknown