A method to exchange alkali light chains on myosin subfragment 1

FEBS Letters

Crasnier, M.¹ 

[1] Department of Biochemistry, University of Birmingham, PO Box 363, Birmingham B15 2TT, England

关键词: Actin;    Myosin;    Myosin subfragment 1;    Alkali light chain;    Hybridization;    S1;    myosin subfragment 1;    S1(A1);    S1(A2);    rabbit muscle myosin subfragment 1 containing either the alkali 1 light chain (A1) or the alkali 2 light chain (A2);    Bicine;    N;    N-bis(2-hydroxyethyl)glycine;    resin SE-53;    sulphoxyethyl cellulose;   

DOI  :  10.1016/0014-5793(87)81268-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exchange of bound alkali light chains on myosin by free alkali light chains is described. It was found that the yield of hybrid obtained was dependent on the incubation time in 4.7 M NH₄Cl at pH 9.5. 60% recovery of S1 (A1) from S1(A2) was obtained using only a 2-fold molar excess of A1 over S1(A2).

【 授权许可】

Unknown