Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB‐2

FEBS Letters

Prieto, Jacqueline³  Patarroyo, Manuel³  Clark, Edward A.¹  Gahmberg, Carl G.²  Kantor, Carmela² 

[1] Department of Microbiology, University of Washington, Seattle, WA 98195, USA;Department of Biochemistry, University of Helsinki, 00170 Helsinki 17, Finland;Department of Immunology, Karolinska Institute, S-104 01, Stockholm 60, Sweden

关键词: LB-2 antibody;    Leukocyte antigen;    Adhesion molecule;   

DOI  :  10.1016/0014-5793(87)81321-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibody LB-2 to a surface antigen on human B cells, lymphoblast, monocytes and vascular endothelial cells largely inhibited adhesion among Epstein Barr virus-immortalized normal B cells (EBV-B) and concanavalin A-stimulated blood mononuclear cells (Con A-BMC) before and after phorbol ester treatment. The antibody inhibited to a lesser extent phorbol ester-induced aggregation of monocytes, U937 cells and fresh BMC and had virtually no inhibitory effect on the adhesion among enriched T cells and granulocytes. A surface glycoprotein band of 84 kDa was obtained from EBV-B cells by immunoprecipitation and gel electrophoresis. Immunological and biochemical studies clearly distinguished this molecule from gp90 and associated glycoproteins which also mediate leukocyte adhesion.

【 授权许可】

Unknown